Alarm bells were ringing at Camp Nou on Sunday when Clement Lenglet was forced off with an ankle problem in the second half of the 4-0 win over Osasuna.
Barcelona are already without injured centre-backs Pique, Araujo and Umtiti, so could not afford to lose another one.
However, coach Ronald Koeman has suggested Lenglet's injury is not too serious, although the club will not know more until Monday.
Koeman said: "I have spoken with the doctor and it seems we will have to wait for tomorrow for tests, but it does not seem that that serious.
"I don't know about Wednesday's game (against Ferencvaros in the Champions League) but I hope it's nothing major."
With so many injuries in defence, Oscar Mingueza has had to step up from the B team. He has done well in his two appearances so far, although Koeman noted he made two cheap mistakes against Osasuna.
"I told him that he played really well, but he lost the ball two times in areas where you cannot lose it," the Dutch coach added.
"We spoke about that. One poor touch can lead to a chance for the opponent. You can have a good game, but two mistakes can cost you the match. He has to learn and improve."